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Flowchart of the inclusion and exclusion process of samples based on quality control criteria and thresholds from Appendix B of the Illumina Local Run Manager TruSight Oncology Comprehensive (EU) Analysis Module Workflow Guide (document no. 200008661; version 3; published July 2022). FFPE, formalin-fixed paraffin embedded; FF, fresh frozen; PCT, percent; MSI, microsatellite instability; MAD, median absolute deviation; CNV, copy number variation; CV, coefficient of variation.

Journal: Experimental and Therapeutic Medicine

Article Title: Comparison of next‑generation sequencing quality metrics and concordance in the detection of cancer‑specific molecular alterations between formalin‑fixed paraffin‑embedded and fresh‑frozen samples in comprehensive genomic profiling with the Illumina® TruSight Oncology 500 assay

doi: 10.3892/etm.2025.12814

Figure Lengend Snippet: Flowchart of the inclusion and exclusion process of samples based on quality control criteria and thresholds from Appendix B of the Illumina Local Run Manager TruSight Oncology Comprehensive (EU) Analysis Module Workflow Guide (document no. 200008661; version 3; published July 2022). FFPE, formalin-fixed paraffin embedded; FF, fresh frozen; PCT, percent; MSI, microsatellite instability; MAD, median absolute deviation; CNV, copy number variation; CV, coefficient of variation.

Article Snippet: The success of TSO 500 DNA and RNA library preparation was assessed based on QC criteria and thresholds from Appendix B of the Illumina Local Run Manager TruSight Oncology Comprehensive (EU) Analysis Module Workflow Guide (document no. 200008661; version 3; published July 2022) ( ).

Techniques: Control, Formalin-fixed Paraffin-Embedded

Median quality control values from Appendix B of the Illumina  Local Run Manager TruSight Oncology Comprehensive (EU) Analysis Module Workflow Guide  (document no. 200008661; version 3; published July 2022).

Journal: Experimental and Therapeutic Medicine

Article Title: Comparison of next‑generation sequencing quality metrics and concordance in the detection of cancer‑specific molecular alterations between formalin‑fixed paraffin‑embedded and fresh‑frozen samples in comprehensive genomic profiling with the Illumina® TruSight Oncology 500 assay

doi: 10.3892/etm.2025.12814

Figure Lengend Snippet: Median quality control values from Appendix B of the Illumina Local Run Manager TruSight Oncology Comprehensive (EU) Analysis Module Workflow Guide (document no. 200008661; version 3; published July 2022).

Article Snippet: The success of TSO 500 DNA and RNA library preparation was assessed based on QC criteria and thresholds from Appendix B of the Illumina Local Run Manager TruSight Oncology Comprehensive (EU) Analysis Module Workflow Guide (document no. 200008661; version 3; published July 2022) ( ).

Techniques: Control, Variant Assay